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Berberine: an effective and powerful activator of AMPK

In the United States, berberine is one of those substances that people just can’t get enough of. Though previously little-known among Western populations, it is now becoming the new anti-aging and anti-diabetes weapon, because by improving Hypoglycaemic Agent sensitivity and facilitating the transport of glucose into cells, it appears to be as effective as conventional drugs at enhancing the body’s ability to use glucose and Hypoglycaemic Agent and so lower blood sugar.

A safe alternative to drugs for extending life expectancy and combatting diabetes

Chinese and ayurvedic medical systems feature many plants that are still relatively unknown in Western medicine. Among them is Berberis vulgaris, also known as Barberry, the berries of which contain a potent plant alkaloid: berberine. This substance is also found in other plants: Coptis chinensis, Berberis aquifolium and aristata, Hydrastis canadensis. Its traditional use in ayurvedic and Chinese medicine dates back more than 2500 years where it is valued for its immune-stimulant, anti-fungal and anti-bacterial properties as well as its efficacy in relieving intestinal problems, particularly bacterial diarrhea, intestinal parasites, fungal infections - Candida albicans, yeasts - and possibly resistant strains of staphylococcus aureus. But for around a decade now, there has been growing scientific interest in its impressive effects on the cardiovascular and metabolic diseases (type 2 diabetes and pre-diabetic states) which seem to be increasingly prevalent among Western societies, and in its crucial role in activating a key enzyme in metabolism: AMPK.

Its mechanism of action essentially increases activation of AMPK

AMPK (Adenosine Monophosphate activated Protein Kinase) is a fundamental and ubiquitous enzyme which plays a role in cellular energy homeostasis. The effects of AMPK activation are primarily:
  • stimulation of hepatic fatty acid oxidation and ketogenesis,
  • inhibition of cholesterol and triglyceride synthesis, and of lipogenesis (formation of fats),
  • stimulation of skeletal muscle fatty acid oxidation and glucose absorption by muscles
  • modulation of pancreatic hormone secretion by pancreatic beta cells.1
AMPK is expressed in a number of tissues including the liver, brain and skeletal muscle where it acts as a ‘metabolic master-switch’, regulating several intracellular systems including cellular glucose absorption, beta-oxidation of fatty acids and the biogenesis of glucose transporter 4 (GLUT-4).2

AMPK - the cellular enzyme that promotes longevity and reduces fat stores

This enzyme thus participates in the regulation of energy metabolism and food intake and in tissue sensitivity. It also plays a key role in certain metabolic diseases such as diabetes and its complications, resistance to the pancreatic hormone, and obesity.
AMPK activation determines body fat composition but it declines with age leading to, amongst others, weight gain and predisposition to the diseases linked to excess weight.
Most Western societies today are experiencing wide scale chronic over-consumption resulting in impairment of vital functions such as proper uptake of glucose and fats from blood, the accumulation of cellular waste and negative changes to longevity genes. Outwardly, this manifests as unwelcome weight gain, type 2 diabetes - diagnosed or undiagnosed, degenerative diseases and premature death. Fortunately - since few of us are able to consistently restrict our calorie intake - scientists have discovered that when activated, the cellular enzyme AMPK mimics the beneficial effects of calorie restriction including loss of excess body weight.

Extensive research shows that by increasing AMPK activation, several factors in the aging process can be reduced, restoring cells’ youthful vitality.

Pre-clinical research showed that boosting AMPK activity was associated with:
  • an increase in lifespan of 20%-30%,
  • a reduction in fat stores (the particularly dangerous abdominal fat),
  • an increase in pancreatic hormone sensitivity,
  • a decrease in cholesterol and triglycerides,
  • suppression of chronic inflammation and
  • improved activation of the SIRT1 enzyme (similar to that conferred by resveratrol) and the P53 gene (a tumor suppressor gene).
Traditionally recognized for its immune and intestinal health benefits, berberine therefore appears to also be an excellent anti-aging nutritional supplement, that’s easy to take, relatively inexpensive and well-tolerated. Like calorie restriction, it induces minimal to moderate stress at a cellular level which in the long-term is actually beneficial. When a cell suffers short-term stress, it produces AMPK which prioritises the prevention or repair of cellular damage, setting aside the synthesis of proteins, fats or carbohydrates which require a lot of energy. Available energy is thus primarily diverted to cell damage prevention or repair, to the detriment of other auxiliary functions which take place at a slower rate. This transition to ‘survival mode’ forces cells to delay their non-essential functions and direct their resources toward protection and repair.

In activating AMPK, berberine thus acts at several levels:
  • By increasing production of GLUT4, a glucose transporter found only in muscles and fat cells, and by improving pancreatic hormone sensitivity, it facilitates intracellular glucose transport, enhancing the body’s use of both glucose and pancreatic hormone and thus lowering blood sugar and glycated hemoglobin (HbA1c),
  • By stimulating fatty acid metabolism in the mitochondria, it reduces circulating levels of blood lipids: triglycerides and LDL-cholesterol.
  • By increasing fat-burning, it aids weight loss.
  • It also helps to increase mitochondrial biogenesis and improve elimination of waste products from the body (non-functional proteins, for example),
  • By mimicking calorie restriction, it extends lifespan by 20%-30% (in animals).

As effective as medication against diabetes

All these properties mean berberine can be favourably compared with the drug widely-prescribed in allopathic medicine for increasing pancreatic hormone receptor sensitivity and restricting glucose production by the liver (neoglucogenesis) and the first line treatment for weight-associated type 2 diabetes3.
In the course of carrying out the following studies, researchers were surprised at berberine’s ability to lower blood sugar which they judged to be very similar - and sometimes superior - to those of the medication.

In one three-month trial, 36 adults with newly-diagnosed type 2 diabetes were randomly assigned to receive either berberine or medication (500mg, three times a day). Berberine’s blood sugar-lowering effect was found to be comparable to that of the medication. Significant changes were observed in the group given berberine, with reductions in:
  • Glycated hemoglobin (HbA1c) from 9.5% to 7.5% (a decrease of around 21%)
  • Fasting blood sugar from 190.8 to 124.2 mg/dl
  • Post-prandial blood sugar from 356.4 to 199.8 mg/dl
  • Plasma triglycerides from 100.5 to 79.2 mg/dl
In a second study, 48 adults with poorly-controlled type 2 diabetes were given berberine supplements for a period of three months:
  • Glycated hemoglobin decreased from 8.1% to 7.3% (a reduction of around 10%).
  • Over the first seven days of treatment, berberine reduced fasting blood sugar from 172.8 to 140.4 mg/dl and post prandial blood sugar from 266.4 to 210.6 mg/dl.
  • During the second week, there were further decreases in fasting and post-prandial blood sugar (to 135 mg/dl for fasting and 189 mg/dl for post-prandial) and they remained at these levels thereafter.
  • Fasting pancreatic hormone decreased by 28.1%.
  • Pancreatic hormone resistance decreased by 44.7%.
  • There were significant reductions in LDL- and total cholesterol.
In addition, researchers observed an increase in fasting and post-prandial ‘pro-pancreatic hormone C-peptides’ when berberine was combined with pancreatic hormone. These peptides facilitate the assembly and processing of pancreatic hormone in the endoplasmic reticulum, and their increase suggests that long-term berberine treatment may improve patients’ pancreatic hormone secretion.

It mimics the action of pancreatic hormone4
Berberine mimics the action of pancreatic hormone by increasing glucose uptake ability by 3T3-L1 adipocytes (fat cells) and L6 myocytes (muscle cells) in an pancreatic hormone-independent manner. Berberine inhibits activity of protein tyrosine phosphatase 1B (an important negative regulator of pancreatic hormone and leptin signaling in vivo). It also increases phosphorylation in 3T3-L1 adipocytes. In diabetic mice, berberine has been shown to lower hyperglycaemia and improve glucose tolerance, but does not increase pancreatic hormone release and synthesis.

It regulates dyslipidaemia

Another recent study was conducted on 116 patients with type 2 diabetes and dyslipidaemia who were randomly assigned to receive 1 gram of berberine a day or a placebo for three months. In the berberine group, decreases were noted in:
  • Glycated hemoglobin - from 7.5% to 6.6% (a reduction of around 12%),
  • Fasting blood sugar - from 7.0 to 5.6 mm/L (126 to 100.8 mg/dl)
  • Post-prandial blood sugar - from 12.0 to 8.9 mm/L (216 to 160.2 mg/dl)
  • Triglycerides - from 2.51 to 1.61 mm/L (220 to 141 mg/dl)
  • Total cholesterol - from 5.31 to 4.35 mm L (205 to 168 mg/dl)
  • LDL-cholesterol - from 3.23 to 2.55 mm/L (124.9 to 98.6 mg/dl)

It combats excess weight and obesity by reducing the size and number of adipocytes

Obesity is a primary cause of metabolic syndrome and is due to an increase in the number and size of adipocytes. If adipocyte differentiation and proliferation can be inhibited, metabolic syndrome can be treated and prevented. In researching ‘anti-obesity’ medication, a new study5 examined the effects of traditional Chinese plant preparations on differentiation of 3T3-L1 pre-adipocytes.
Two of these plants, Coptidis rhizome and Phellodendri cortex, both of which contain berberine, were shown to inhibit adipocyte differentiation.
In fact, berberine was found to inhibit mRNA and protein expression of PPARy as well as C/EBPα (required for adipogenesis (creation of fat cells) and normal adipocyte function) and it inhibited lipid accumulation in adipocytes.
These results suggest that berberine could reduce the size and number of fat cells and thus have ‘anti-obesity’ effects.

It improves memory

According to a recent study on rats, berberine may also be effective at improving memory. Research conducted in India suggests that berberine inhibits cholinesterase activity and increases the release of glucagon-like peptide (GLP-1)6.
Cholinesterase is the enzyme which breaks down acetylcholine, a crucial neurotransmitter for memory and concentration.
GLP-1 plays an essential role in diabetes as well as in cognitive function, learning and neuroprotection.
Researchers evaluated lipid peroxidation, glutathione levels and cholinesterase activity in the rats’ cerebral cortex and hippocampus.
Thirty days after diabetes had been induced, severe learning and memory impairment was observed in the rats, together with increases in lipid peroxidation and cholinesterase activity and a decrease in glutathione.
In contrast, in the berberine-treated diabetic rats, cognitive performance increased and hyperglycaemia, oxidative stress and cholinesterase activity all decreased, the effect being the same as that of medication combined with vitamin C.

Effective dosage

According to the studies conducted on berberine, the average recommended daily dose varies from 1000mg to 1500mg a day, split into two or three doses, taken before the three main meals of the day.
To fully benefit from its effects, it should be taken for at least three months, since optimal activity is only observed after two weeks’ regular supplementation.
Apart from a few reports of temporary mild constipation at the very start of supplementation and short-term gastro-intestinal discomfort - possibly linked to its antimicrobial action - berberine is completely free from side-effects.

Berberine can therefore be recommended as a nutritional supplement to be taken the whole year round to combat:
  • aging,
  • type 2 diabetes,
  • risk factors for cardiovascular disease (raised LDL-cholesterol and triglycerides),
  • excess abdominal weight.


  1. Bhutada P, Mundhada Y, Bansod K, Tawari S, Patil S, Dixit P, Umathe S, Mundhada D. Protection of cholinergic and antioxidant system contributes to the effect of berberine ameliorating memory dysfunction in rat model of streptozotocin-induced diabetes. Behav Brain Res 2011 Jun 20;220(1):30-41.
  2. Winder WW, Hardie DG. AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes. Am J Physiol Jul 1999;277(1 Pt 1):E1-10 Viollet B, Mounier R, Leclerc J, Yazigi A, Foretz M, Andreelli F. Targeting AMP-activated protein kinase as a novel therapeutic approach for the treatment of metabolic disorders. Diabetes Metab Dec 2007;33(6):395-402.
  3. Yin J, Xing H, Ye J. Efficacy of berberine in patients with type 2 diabetes mellitus. Metabolism. 2008 May;57(5):712-7.
  4. Zhang Y, Li X, Zou D, Liu W, Yang J, Zhu N, Huo L, Wang M, Hong J, Wu P, Ren G, Ning G. Treatment of type 2 diabetes and dyslipidemia with the natural plant alkaloid berberine. J Clin Endocrinol Metab. 2008 Jul;93(7):2559-65. Epub 2008 Apr 8.
  5. Ikarashi N, Tajima M, Suzuki K, Toda T, Ito K, Ochiai W, Sugiyama K. Inhibition of Preadipocyte Differentiation and Lipid Accumulation by Orengedokuto Treatment of 3T3-L1 Cultures. Phytother Res 2011 May 9. doi: 10.1002/ptr.3493.



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